To preserve the integrity of the proteins during electrophoresis, it is important to keep the apparatus cool and to minimize denaturation and proteolysis. Thus, this technique can be used for the preparation of purified, active proteins.įollowing electrophoresis, proteins can be recovered from a native gel by passive diffusion or electroelution. In addition, some proteins retain their enzymatic activity (function) after separation by Native-PAGE. Since no denaturants are used in Native-PAGE, subunit interactions within a multimeric protein are usually preserved and information about the quaternary structure can be obtained. Thus, native-PAGE separates proteins according to both their charge and mass. Small proteins face only a small frictional force, while larger proteins face a greater frictional force. At the same time, the friction force of the gel matrix creates a sieving effect by regulating the movement of the proteins according to their size and three-dimensional shape. The higher the negative charge density (more charge per molecular mass), the faster a protein will migrate. Electrophoretic migration occurs because most proteins carry a net negative charge in alkaline working buffers. In Native-PAGE, proteins are separated according to the net charge, size, and shape of their native structure. These reference protein sets are called mass markers or molecular weight markers (MW markers), protein ladders, or size standards, and they are commercially available in a variety of forms. When a set of proteins of known mass are run alongside samples in the same gel, they provide a reference from which the mass of the sample proteins can be determined. Proteins from almost any source are readily solubilized by SDS, so the method is generally applicable. The simplicity and speed of this method, as well as the fact that only microgram amounts of protein are required, have made SDS-PAGE the most widely used method for determining the molecular mass in a polypeptide sample. As a result, proteins pass through the gel strictly by polypeptide size, with little effect from compositional differences. The intrinsic charges of the polypeptide are negligible compared to the negative charges provided by the bound detergent, so that the SDS-polypeptide complexes have essentially the same negative charge and shape. Under these conditions, most polypeptides bind SDS at a fixed weight ratio (1.4 g SDS: 1 g polypeptide). By heating the protein sample to 70-100☌ in the presence of excess SDS and thiol reagent, the disulfide bonds are cleaved and the protein completely dissociates into its subunits. SDS denatures proteins by wrapping the polypeptide backbone. In SDS-PAGE, the gel is poured into a buffer containing sodium dodecyl sulfate (SDS), an anionic detergent. Native-PAGE with SDS-PAGE (denaturing) SDS-PAGE TEMED (N,N,N',N'-tetramethylenediamine) catalyzes the polymerization reaction by promoting the production of free radicals by APS. Polyacrylamide gels are prepared by mixing acrylamide with bisacrylamide to form a cross-linked polymer network when the polymerizing agent, ammonium persulfate (APS), is added. Polyacrylamide is the material of choice for preparing electrophoretic gels to separate proteins by size. Polyacrylamide has a smaller pore size and is ideal for separating most proteins and smaller nucleic acids. Agarose has a large pore size and is suitable for separating nucleic acids and large protein complexes. These matrices act as porous media and act as molecular sieves. Polyacrylamide and agarose are two support matrices commonly used in electrophoresis. The mobility of a molecule through an electric field depends on factors such as field strength, net charge on the molecule, size and shape of the molecule, ionic strength, and properties of the matrix (eg, viscosity, pore size) through which the molecule passes. Most biological molecules carry a net charge at any pH other than their isoelectric point and migrate at a rate proportional to their charge densities. Both proteins and nucleic acids can be separated by electrophoresis, a simple, fast and sensitive analytical tool. Protein electrophoresis is a standard laboratory technique in which charged protein molecules are transported through a solvent by an electric field.
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